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KMID : 1094020150320020154
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Journal of Veterinary Clinics
2015 Volume.32 No. 2 p.154 ~ p.157
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Development of a Panel of Multiplex Real-Time Polymerase Chain Reaction Assays for Simultaneous Detection of Canine Enteric Bacterial Pathogens
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Jang Hye-Jin
Kang Hyo-Min
Na Ki-Jeong
Han Jae-Ik
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Abstract
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A major cause of diarrhea in a dog is an infection with bacteria which include Salmonella spp.,Campylobacter (C.) spp., and Clostridium (Cl.) spp.. It is fastidious to identify these bacteria by the culture. The purposeof this experiment is to devise the method for detecting Cl. perfringens, C. jejuni, C. coli, and Salmonella spp. withrapid and high sensitivity. The fecal samples collected from 71 normal and 66 diarrheic dog feces were used to comparethe prevalence of the enteric pathogens and to develop a multiplex real-time polymerase chain reaction (PCR) assayfor clinical use. Detection of Cl. perfringens, C. coli, and C. jejuni in diarrhea feces was higher than normal feces. A developed multiplex real-time PCR is useful for determining the presence and quantity of pathogen-specific or otherunique sequences with in a fecal sample.
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KEYWORD
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PCR, Clostridium perfringens, Campylobacter jejuni, Campylobacter coli, Salmonella spp
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